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Biotechnology Letters
Publisher: Springer
Netherlands
ISSN: 0141-5492
(Paper) 1573-6776 (Online)
DOI: 10.1023/A:1018394616153
Issue:
Volume 19, Number 4
Date:
April 1997
Pages: 331
- 334 |
Site-specific
recombination system from Tn1000(   ) stabilises recombinant plasmids in Escherichi
coli Marina Bellani1,
Clara Nudel2 and Carmen Sanchez
Rivas2
| (1) |
Facultad de Ciencias Exactas
y Naturales, Depto. Quimica Bioligica, Ciudad
Universitaria, pabellon 2 piso 4,
Argentina |
| (2) |
Facultad de Farmacia y
Bioqu'mica, Biotecnologia, Junin 956 (1113), Universidad
de Buenos Aires, Buenos Aires,
Argentina |
Abstract The
resolution system from Tn1000 was cloned into a pBR322
recombinant plasmid carrying E. coli threonine operon. The
resulting plasmid displayed a significant increase in
segregational stability with respect to the original plasmid,
either in a rec or a rec E. coli strain (100% of
plasmid-containing cells versus 64% and 1% respectively). The
Tn1000 resolution system proved to be responsible for the
stabilising effect exerted by the transposon when present in
recombinant plasmids.
References
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